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FAQ
Reasons for non-growth or low values when using lyophilized strains?
a) Transportation problems: lyophilized strains were not stored below -18℃ in time after delivery to the destination, resulting in prolonged exposure to room temperature or high temperature.
b) Operational problems: the sample amount was wrong after lyophilized strains were reconstituted; the temperature of the culture medium was too high when pouring method was used; the lyophilized strains were not used as soon as possible after reconstituted and were not stored in the refrigerator at 2-8℃ for 24h.
c) Problems of culture medium: some domestic culture medium may cause Pseudomonas aeruginosa, E. coli and so on to be difficult to grow; selective culture medium is used for culture.
d) Problems with culture conditions: insufficient incubation time; aerobic bacteria (e.g. Pseudomonas aeruginosa) are incubated with sealing treatment, or anaerobic bacteria (e.g. Clostridium perfringens) are not anaerobically treated; the medium is close to the wall of the incubator.
b) Operational problems: the sample amount was wrong after lyophilized strains were reconstituted; the temperature of the culture medium was too high when pouring method was used; the lyophilized strains were not used as soon as possible after reconstituted and were not stored in the refrigerator at 2-8℃ for 24h.
c) Problems of culture medium: some domestic culture medium may cause Pseudomonas aeruginosa, E. coli and so on to be difficult to grow; selective culture medium is used for culture.
d) Problems with culture conditions: insufficient incubation time; aerobic bacteria (e.g. Pseudomonas aeruginosa) are incubated with sealing treatment, or anaerobic bacteria (e.g. Clostridium perfringens) are not anaerobically treated; the medium is close to the wall of the incubator.
What are the advantages of microdome QC strains?
1、Accurate cfu number of known strains, reduce the failure rate of the experiment, avoid all kinds of bacterial suspension dilution and personnel operation caused by the failure.
2, the same batch of quality control strains with stable cfu counts, reducing the differences between samples.
3, greatly reduce the experimental preparation time, easy to use and reduce costs, the use of quality control strains only need 10s re-dissolution and personnel have a certain aseptic operation ability and the ability to use the pipette gun can be, greatly reducing the cost of personnel training and the use of raw materials costs.
4, no traceability problems, can be traced back to CMCC or ATCC strain conservation center.
5、Stable bacterial volume, valid for up to two years.
2, the same batch of quality control strains with stable cfu counts, reducing the differences between samples.
3, greatly reduce the experimental preparation time, easy to use and reduce costs, the use of quality control strains only need 10s re-dissolution and personnel have a certain aseptic operation ability and the ability to use the pipette gun can be, greatly reducing the cost of personnel training and the use of raw materials costs.
4, no traceability problems, can be traced back to CMCC or ATCC strain conservation center.
5、Stable bacterial volume, valid for up to two years.
What is the difference between commercial derived strains and standard strains?
From a technical point of view, standard strains are purchased from nationally recognized institutions or foreign strain preservation institutions, and are lyophilized powders of 0-generation strains, which need to be resuscitated, passed on, identified and preserved before use, and need to be activated one day before use, and need to be counted and quantified after gradient dilution.
While the commercial derived strains are derived from the standard strains, usually in lyophilized form, generally 2-5 generations of strains, which is characterized by no need for activation, generation and preservation, ready to use, without the need for advance activation and gradient dilution, in accordance with the instructions provided by the manufacturer to get the required number of bacterial fluid, greatly saving the workload of microbiology laboratory staff.
In terms of document management, when using standard strains, from standard strains to standard reserve strains, to working strains, this series of use process should have the corresponding procedural documents and records, and the relevant personnel must carry out the necessary training, with complete training records; while the use of commercial strains only need to be provided by the manufacturer of the quality inspection report and traceability report, to ensure that it is equivalent to the standard strains of all relevant characteristics, and to ensure that it can be used as the standard strains, and also to ensure that it can be used as the standard strains. The use of commercially derived strains only requires the manufacturer to provide quality inspection reports and traceability reports to ensure that they are equivalent to the standard strains in all relevant characteristics, and at the same time ensure their traceability.
In addition, compared with commercial strains, the use of standard strains requires a relatively large investment in hardware facilities and consumables, and the cost is not lower than that of commercial strains.
In the field of microbiological testing, there is no obvious difference between the application scope of commercial strains and standard strains, which can be used for sensitivity test, growth promotion test, sterility test, medium suitability test, control bacteria test, etc. in pharmaceutical testing; quantitative and qualitative control in food testing, medium quality control, etc.; and microbiological quality control of cosmetic testing.
In terms of document management, when using standard strains, from standard strains to standard reserve strains, to working strains, this series of use process should have the corresponding procedural documents and records, and the relevant personnel must carry out the necessary training, with complete training records; while the use of commercial strains only need to be provided by the manufacturer of the quality inspection report and traceability report, to ensure that it is equivalent to the standard strains of all relevant characteristics, and to ensure that it can be used as the standard strains, and also to ensure that it can be used as the standard strains. The use of commercially derived strains only requires the manufacturer to provide quality inspection reports and traceability reports to ensure that they are equivalent to the standard strains in all relevant characteristics, and at the same time ensure their traceability.
In addition, compared with commercial strains, the use of standard strains requires a relatively large investment in hardware facilities and consumables, and the cost is not lower than that of commercial strains.
In the field of microbiological testing, there is no obvious difference between the application scope of commercial strains and standard strains, which can be used for sensitivity test, growth promotion test, sterility test, medium suitability test, control bacteria test, etc. in pharmaceutical testing; quantitative and qualitative control in food testing, medium quality control, etc.; and microbiological quality control of cosmetic testing.
Difference between disinfection and sterilization?
Disinfection and sterilization are two concepts. Sterilization, disinfection and sanitization are three often misused terms that must be distinguished. Sterilization and sterilization are absolutist terms. Sterilization is the killing or inactivation of all life forms; decontamination is the destruction or inactivation of the infection of disease-causing microorganisms, but is not necessarily effective when applied to bacterial spores. Sterilization is the process of sterilizing aseptic preparations, etc., and commonly used methods include: moist heat sterilization, which can be used to express the effect of sterility assurance value SAL (Sterility Assurance Level); dry heat sterilization; decontamination filtration; radiation sterilization; and ethylene oxide sterilization. Sterilization, on the other hand, can be described as reducing the number of microorganisms to a safe or relatively safe level that is compatible with the regulations for use and the purpose of use. Anti-microbial agent is a general term for an agent used to inhibit the reproduction of microorganisms or to destroy microorganisms, and it includes:
(1) Disinfectant A chemical agent that eliminates microbial contamination in the form of cellular reproduction;
(2) Sterilizing agent A chemical agent that kills all microbial life forms within an inanimate environment;
(3) Sterilizing agent An antimicrobial agent used to eliminate pathogens on inanimate objects;
(4) Bactericides May be classified according to specific roles: those that kill bacteria are called bactericides; those that kill molds are called mycocides; those that kill viruses are called virucides; and those that kill spores are called sporicides;
(5) Bacteriostatic agents are only chemical agents that inhibit the growth of microorganisms.
Sterilization and disinfection are therefore not interchangeable terms, nor does disinfectant by definition imply that it is a sterilant. Disinfection is the elimination of contamination by live microorganisms taken from the cells of the reproductive form, but it does not guarantee sterilization.
(1) Disinfectant A chemical agent that eliminates microbial contamination in the form of cellular reproduction;
(2) Sterilizing agent A chemical agent that kills all microbial life forms within an inanimate environment;
(3) Sterilizing agent An antimicrobial agent used to eliminate pathogens on inanimate objects;
(4) Bactericides May be classified according to specific roles: those that kill bacteria are called bactericides; those that kill molds are called mycocides; those that kill viruses are called virucides; and those that kill spores are called sporicides;
(5) Bacteriostatic agents are only chemical agents that inhibit the growth of microorganisms.
Sterilization and disinfection are therefore not interchangeable terms, nor does disinfectant by definition imply that it is a sterilant. Disinfection is the elimination of contamination by live microorganisms taken from the cells of the reproductive form, but it does not guarantee sterilization.
What are the causes of strain contamination?
In the process of microbiological testing, contamination by stray bacteria often occurs. Therefore, we must attach great importance to standardize the operation, pay attention to each operational link, as far as possible not to be contaminated.
(1) culture medium contamination
Composition of the culture medium of the various raw materials, itself mixed with different degrees of stray bacteria and fungal spores. If disinfection and sterilization is not thorough, cultivation for about 5 days, in the media surface and the internal will occur at the same time the stray bacteria.
(2) Operation contamination
In the case that the culture medium has been thoroughly sterilized, it is found that the surface of the transferred medium (including around the seed block) is contaminated with stray bacteria, which means that the inoculation room (box), utensils, the surface of the vials of stray bacteria and the operator's hands and uniforms are not well disinfected, or the test operation procedures are violated.
(3) Environmental pollution
Due to the culture room disinfection is not complete, or biological safety cabinet efficient leakage, or petri dishes, culture bottles are not sterile, often resulting in contamination of stray bacteria after cultivation.
(4) Contamination of the strain itself
Due to the impurity of the strain itself, contaminated with stray bacteria. When they are transferred, it will cause contamination.
(1) culture medium contamination
Composition of the culture medium of the various raw materials, itself mixed with different degrees of stray bacteria and fungal spores. If disinfection and sterilization is not thorough, cultivation for about 5 days, in the media surface and the internal will occur at the same time the stray bacteria.
(2) Operation contamination
In the case that the culture medium has been thoroughly sterilized, it is found that the surface of the transferred medium (including around the seed block) is contaminated with stray bacteria, which means that the inoculation room (box), utensils, the surface of the vials of stray bacteria and the operator's hands and uniforms are not well disinfected, or the test operation procedures are violated.
(3) Environmental pollution
Due to the culture room disinfection is not complete, or biological safety cabinet efficient leakage, or petri dishes, culture bottles are not sterile, often resulting in contamination of stray bacteria after cultivation.
(4) Contamination of the strain itself
Due to the impurity of the strain itself, contaminated with stray bacteria. When they are transferred, it will cause contamination.
What are some common methods of preserving microorganisms?
Common microbial preservation methods include slant cryopreservation, glycerol tube preservation, magnetic bead preservation, and vacuum freeze-drying preservation.
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